Histopathology

Gelatin is a hydrocolloid and is rich in glycine, proline and hydroxyproline, which impart structural stability. It is synthesized from the alkaline digestion of collagen from bovine skin and is referred as type B. It has wide applications in food industry.

Eosin is tetra bromofluorescein (a substituted xanthene), a red acidic dye and fluorochrome. The dye is very soluble in ethyl alcohol. Eosin, 2% w/v is the most commonly used as counterstain for hematoxylin and it is also used for monochrome staining of bacteria.