Bacteriology

Description
C.L.E.D. Agar w/Bromo Thymol Blue is recommended for use in urinary bacteriology. This medium promotes the growth of all urinary pathogens. Lactose is the fermentable sugar. Bromo thymol blue is the pH indicator which turns yellow at acidic pH. Colonies grow as blue or yellow due to fermentation of lactose.

Bile Esculin Agar is recommended for isolation and presumptive identification of group D Streptococci from food and pharmaceutical products. Enterococci and Group D Streptococci hydrolyse esculin to esculetin and dextrose, which reacts with ferric citrate producing brownish black precipitate. Identification of enterococci and differentiation of Group D strptococci from non Group D can thus be performed using this agar medium.

Recommended for isolation and enumeration of Staphylococci in food and other material since it allows a good differentiation of coagulase positive strains. the use of Baird-Parker Agar was officially adopted by AOAC International and is recommended in the USP for use in the performance of Microbial Limit Tests. Recently, ISO committee has also recommended this medium for the isolation and enumeration of Staphylococci. The isolation and enumeration of coagulase positive Staphylococci from food and clinical samples

Agar Agar, Type I is recommended for preperation of culture media for microbiological analysis.

This media also known as RMW Agar is used for isolation, enumeration and identification of Lactobacilli from foodstuffs and clinical specimens. Low pH of the medium and addition of acetic acid makes the medium selective for Lactobacilli inhibiting other bacterial flora. It is recommended that the plates should be incubated at 30°C for 5 days or at 37°C for 3 days in an atmosphere of 95% hydrogen and 5% carbon dioxide. If this is not possible, overlay the inoculated plates with a second layer of the agar before incubation. All colonies should be checked by gram staining and by catalase test before further identification. The salt in the formulation makes the medium unsuitable for isolation of dairy Lactobacilli. e.g. L. lactis, L. bulgaricus and L. helveticus.

PPotassium hydroxide (KOH) is a hygroscopic, water soluble base synthesized by the electrolysis of potassium chloride solution. It is the raw material for the synthesis of many potassium compounds.

Benedict's reagent is used to test for the presence of reducing sugars. Benedict’s quantitative reagent is a modification of qualitative aspects. It contains copper sulphate-sodium carbonate. It also contains potassium thiocyanate and small amount of potassium ferricyanate.The reduction of Cu3+ ions by sugar is a non-stoichiometric equation and is only constant over a small range of sugar concentration.