Culture media

Luria Bertani Broth, Miller is slightly different with double amount of sodium chloride as compared to original media described by Lennox. Luria Bertani Broth, Miller is used for the cultivation and maintenance of recombinant strains of Escherichia coli and may be used for routine cultivation of not particularly fastidious microorganisms. LB with or without antibiotics can also be used for selective culture of antibiotic-resistant strains of E. coli. Used for non-selective cultivation of E. coli strains for cloning, production of DNA, plasmid DNA and recombinant proteins.

This medium is recommended for selective isolation and differentiation of coliform organisms and other enteric pathogens screening. Based on lactose fermentation with neutral red as indicator. Detection of Enterobacteriaceae and Coliforms . Selective medium which contains Bile salt mixture and crystal violet which inhibits gram positive bacteria.

Description
This medium is used for maintaining microorganisms, cultivating fastidious organisms by enriching with serum or blood and are also used for purity checking prior to biochemical or serological testing.

Yeast Extract Agar is recommended for plate count of microorganisms in water. This medium is highly nutritive. The organisms grows luxuriantlly on the media. Yeast extract and peptone provide nitrogenous compounds, vitamin B complex and other growth nutrients.The plates should be selected containing 30-300 colonies.

Phosphate Buffered Saline, pH 7.2 is recommended for preparation of dilutions and blanks for the examination of water, dairy products, foods, utensils and other specimens. This solution gives a pH of 7.2.

Boric Acid Solution is used as standard reagent for analytical purpose.

EDTA, 1M is standard solution used for volumetric titration in analytical chemistry.

Giemsa stain is a mixture of methylene blue, eosin, and azure B. It is specific for the phosphate groups of DNA and attaches itself to adenine-thymidine rich regions. It is a high contrast polychromatic stain. It is used as a standard stain for differential staining of blood and bone marrow smears. It stains different cells in different colour. Giemsa is commonly used stain for G-banding of chromosomes. In G-banding studies, heterochromatic region of chromosomes is stained dark(G-bands) with giemsa whereas euchromatic regions are stained light(R-bands). This helps in identification of chromosomal aberrations.