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Eosin is tetra bromofluorescein (a substituted xanthene), a red acidic dye and fluorochrome. The dye is very soluble in ethyl alcohol. Eosin, 2% w/v is the most commonly used as counterstain for hematoxylin and it is also used for monochrome staining of bacteria.

both the dyes.

Decolourizer is one reagent used in this process to provide the color differentiation. A decolourizing solution is used to remove the Primary Stain from cells which bind it weakly and then the counterstain (like safranin) is used to provide a colour contrast in those cells that are decolourized.

Negative staining method permits visualization of the usually transparent and unstainable capsule of many organisms, most importantly Cryptococcus neoformans. Nigrosin consists of a suspension of fine particles of carbon. These form a dark background.