Antibiotic Sensitivity disc are used to determine the effectivness of an antibiotic against a microorganism as a guide to antibiotic therapy. The disc consist of a specific concentration of an antibiotic in mcg which is tested against a microorganism of a standard density, swabbed uniformly on an appropriate media following overnight incubation. Cefixime is impregnated on a filter paper disc of specified concentration. Both sides of the disc are printed with CFM and concentration 5 mcg for easy identification.

Antibiotic Sensitivity disc are used to determine the effectivness of an antibiotic against a microorganism as a guide to antibiotic therapy. The disc consist of a specific concentration of an antibiotic in mcg which is tested against a microorganism of a standard density, swabbed uniformly on an appropriate media following overnight incubation. Amikacin is impregnated on a filter paper disc of specified concentration. Both sides of the disc are printed with AK and concentration 30mcg for easy identification.

This medium detects urease activity by all rapidly urease-positive Proteus organisms and also by other members of Enterobacteriaceae that exhibits a delayed urease reaction. Prolonged incubation may cause alkaline reaction in the medium. A medium without urea serves as negative control to rule out false positive results. Also, all urea test media rely on the alkalinity formation and so they are not specific for determining the absolute rate of urease activity. The utilization of proteins may raise the pH to alkalinity due to protein hydrolysis and excess of amino acids liberation results in false positive reaction.

Originally proposed by Sulkin and Willett and modified by Hajna for identifying Enterobacteriaceae . This medium complies with the recommendation of APHA, for the examination of meat and food products , for the examination of milk and dairy products and for microbial limit test for confirming the presence of Salmonellae and in the identification of gram-negative bacilli. The reactions can be interpreted as follows: Alkaline slant / acid butt- only glucose fermented, Acid slant / acid butt- dextrose and sucrose fermented or dextrose and lactose fermented or all the three sugars, dextrose,lactose and sucrose fermented, Bubbles or cracks present-gas production Black precipitate present-H2S gas production