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M17 Broth is recommended for cultivation of lactic Streptococci and their bacteriophages. It is usedfor the cultivation and enumeration of lactic Streptococci and their bacteriophages. This medium is a modification of M16 Medium.

Lactobacillus MRS Agar (MRS Agar)Recommended for the isolation and enumeration of lactic acid bacteria from meat and meat products. The composition and performance criteria of this medium are as per the specifications laid down in ISO 1995, Draft ISO/DIS 13720:2010. Peptone, HM Extract B, yeast extract , dextrose provides carbon and nitrogen sources for lactobacilli growth. Sodium acetate and ammonium citrate inhibit Streptococci, moulds and many other microorganisms. Magnesium sulphate and manganese sulphate provide essential ions for multiplication of lactobacilli. Phosphates provide good buffering action in the media. Polysorbate 80 supplies fatty acids required for the metabolism of Lactobacilli.

It a combination of the lead acetate medium described by Kligler and Russels Double Sugar Agar and is used as a differentiation medium for typhoid, dysentery and allied bacilli. It differentiates lactose fermenters from the non-fermenters, Salmonella Typhi from other Salmonellae and also Salmonella Paratyphi A from Salmonella Scottmuelleri and Salmonella Enteritidis

Gelatin is a hydrocolloid and is rich in glycine, proline and hydroxyproline, which impart structural stability. It is synthesized from the alkaline digestion of collagen from bovine skin and is referred as type B. It has wide applications in food industry.

Recommended for rapid cultivation of aerobes as well as anaerobes including microaerophiles by adding a reducing agent and small amount of agar. The USP, BP, EP and AOAC have recommended the media for sterility testing of antibiotics, biologicals and foods and for determining the phenol coefficient and sporicidal effect of disinfectants. it is intended for the examination of clear liquid or water-soluble materials. FTM is also routinely used to check the sterility of stored blood in blood banks.

DNase Test Agar Base is recommended for detection of deoxyribounclease activity of bacteria and fungi, and especially for identification of pathogenic Staphylococci. Addition of toluidine blue in the media is used for differentiation and identification of non-pigmented Serratia species isolated from clinical sources that might be improperly identified as Enterobacter and Klebsiella species. Positive DNase activity is visualized as clear zones (around colonies) when the plates were flooded with 1 N hydrochloric acid.

This medium is used as the base for the media containing blood and for selective media formulations in which different combinations of antimicrobial agents are used as additives. It is also selective for the isolation of Haemophilus species from clinical specimens, especially from upper respiratory tract.

Recommended for isolation and enumeration of Staphylococci in food and other material since it allows a good differentiation of coagulase positive strains. the use of Baird-Parker Agar was officially adopted by AOAC International and is recommended in the USP for use in the performance of Microbial Limit Tests. Recently, ISO committee has also recommended this medium for the isolation and enumeration of Staphylococci. The isolation and enumeration of coagulase positive Staphylococci from food and clinical samples

This media also known as RMW Agar is used for isolation, enumeration and identification of Lactobacilli from foodstuffs and clinical specimens. Low pH of the medium and addition of acetic acid makes the medium selective for Lactobacilli inhibiting other bacterial flora. It is recommended that the plates should be incubated at 30°C for 5 days or at 37°C for 3 days in an atmosphere of 95% hydrogen and 5% carbon dioxide. If this is not possible, overlay the inoculated plates with a second layer of the agar before incubation. All colonies should be checked by gram staining and by catalase test before further identification. The salt in the formulation makes the medium unsuitable for isolation of dairy Lactobacilli. e.g. L. lactis, L. bulgaricus and L. helveticus.