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Blood Agar Base w/ Nalidixic Acid is recommended for differentiation of haemolytic activity of Streptococci.It is a highly nutritive medium. Microorganisms producing haemolysin give visible haemolytic zones on this medium. Blood Agar Base is modified with addition of Nalidixic acid which acts as an inhibitor for the accompanying flora and to support the growth of Staphylococci, haemolytic Streptococci and Enterococci when supplemented with 5% blood. Nalidixic acid blocks the DNA replication of susceptible bacteria and acts against many Gram-negative bacteria.

Clostridium Difficile Agar Base is recommended for selective isolation of Clostridium difficile from food and certain pathological specimens.

Luria Bertani Broth, Miller is slightly different with double amount of sodium chloride as compared to original media described by Lennox. Luria Bertani Broth, Miller is used for the cultivation and maintenance of recombinant strains of Escherichia coli and may be used for routine cultivation of not particularly fastidious microorganisms. LB with or without antibiotics can also be used for selective culture of antibiotic-resistant strains of E. coli. Used for non-selective cultivation of E. coli strains for cloning, production of DNA, plasmid DNA and recombinant proteins.

This medium is recommended for selective isolation and differentiation of coliform organisms and other enteric pathogens screening. Based on lactose fermentation with neutral red as indicator. Detection of Enterobacteriaceae and Coliforms . Selective medium which contains Bile salt mixture and crystal violet which inhibits gram positive bacteria.

Description
This medium is used for maintaining microorganisms, cultivating fastidious organisms by enriching with serum or blood and are also used for purity checking prior to biochemical or serological testing.

Yeast Extract Agar is recommended for plate count of microorganisms in water. This medium is highly nutritive. The organisms grows luxuriantlly on the media. Yeast extract and peptone provide nitrogenous compounds, vitamin B complex and other growth nutrients.The plates should be selected containing 30-300 colonies.

Antibiotic Sensitivity disc are used to determine the effectivness of an antibiotic against a microorganism as a guide to antibiotic therapy. The disc consist of a specific concentration of an antibiotic in mcg which is tested against a microorganism of a standard density, swabbed uniformly on an appropriate media following overnight incubation. Colistin (Methane Sulphonate) is impregnated on a filter paper disc of specified concentration. Both sides of the disc are printed with CL and concentration 25 mcg for easy identification.

A combination of 24 tests for the identification o