This medium is highly recommended by APHA for the selective isolation of V. cholerae and V. parahaemolyticus. Enrichment in Alkaline Peptone Water (M618), followed by isolation on TCBS Agar is routinely used for isolation of V.cholerae.

Streptococcus Selection Agar is recommended for selective isolation and enumeration of all types of Streptococci, including group A beta haemolytic strains. Sodium azide, sodium sulphite inhibits gram-negative rods and the crystal violet suppresses Staphylococci. Growth of coliforms, Proteus, Pseudomonas and Bacillus species is markedly suppressed in this medium.

These media are used for the differentiation between Enterobacteriaceae and the members of aerogenes group on the basis of citrate utilization as sole carbon source by IMViC tests.

Selenite Cystine Broth Base w/o Biselenite is recommended as a selective enrichment media for Salmonella and possibly Shigella sonnei from faeces, urine, water and foodtuffs. It is useful for detecting Salmonella in the nonacute stages of illness when organisms occur in the faeces in low numbers and for epidemiological studies to enhance the detection of low number of organisms from asymptomatic or convalescent patients.

This medium is used as a selective enrichment medium for Salmonellae species from food, animal feeding stuffs, pharmaceutical products. This medium can also be used in direct enrichment of samples containing low inoculum

for determining plate counts of microorganisms in milk and dairy products by pour plate technique.it is also used for the estimation of the number of live heterotrophic bacteria in water.

This medium is used for maintaining microorganisms, cultivating fastidious organisms by enriching with serum or blood and are also used for purity checking prior to biochemical or serological testing.

Standard technique for staining reticulocytes is the new methylene blue staining. New methylene blue stains intracellular RNA blue in colour against pink stained RBCs. Depending on the stage of the reticulocyte the blue colour may be distributed in a general pattern(early reticulocytes) or punctate pattern(late reticulocyte). Cells with clear, blue cytoplasmic granules which can be seen without fine focusing are to be regarded as reticulocytes of maturation stage IV. From visual examination of a peripheral blood film so stained, the number of reticulocytes among 1000 reticulocytes is found and expressed as a percentage. This is termed the reticulocyte count. TCL115 is a reticulocyte staining solution formulated to contain new methylene blue for staining reticulocyte in blood smear.

MIU Medium Base is recommended for detection of motility, urease and indole production in single tube. Motility and urease reactions are read before testing Indole production. Motile organisms show either diffused growth or turbidity extending away from stab inoculation line while nonmotile organisms grow along the stabline. Organisms that utilize urea, produce ammonia which makes the medium alkaline, showing pink-red colour by change in the phenol red indicator. Indole is produced from tryptophan present in tryptone. The indole produced combines with the aldehyde present in the Kovac's reagent to form a red complex.